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Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
 

Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs

Article DOI: 10.3791/53782-v • 09:47 min • June 17th, 2016
June 17th, 2016 •
1,2, 1,3,4, 1,2,5
1Sinclair Centre for Regenerative Medicine, Ottawa Hospital Research Institute, 2University of Ottawa, 3Department of Neonatology and Pediatric Critical Care Medicine, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, 4DFG Research Center and Cluster of Excellence for Regenerative Therapies (CRTD), Technische Universität, Dresden, 5Children's Hospital of Eastern Ontario Research Institute

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This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.

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Keywords: CD146+ Resident Lung Mesenchymal Stromal Cells Rat Lungs Enzymatic Digestion Density Gradient Separation Plastic Adherence Bead Sorting Pulmonary Disease Immune Modulation Tissue Repair Trachea Bronchi CPDA Anticoagulant PBS Dulbecco's PBS Sodium Pyruvate Glucose Scalpel Mincing Enzyme Digestion EDTA FBS Cell Strainer
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