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Journal / Neuroscience / Designing, Packaging, and Delivery of High Titer…
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Neuroscience

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JoVE Journal Neuroscience
Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
 

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Article DOI: 10.3791/53783-v • 11:28 min • May 23rd, 2016
May 23rd, 2016 •
1, 1, 1, 1, 1
1Department of Physiology and Neurobiology, Geisel School of Medicine at Dartmouth College

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The CRISPR/Cas9 system offers the potential to make targeted genome editing accessible and affordable to the scientific community. This protocol is intended to demonstrate how to create viruses that will knockout a gene of interest using the CRISPR/Cas9 system, and then inject them stereotaxically into the adult mouse brain.

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Keywords: CRISPR Retrovirus Lentivirus Stereotaxic Injection Neuroscience Gene Manipulation Guide RNA Plasmid Design Cell Culture Transfection HEK293T Cells
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