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JoVE Journal Immunology and Infection
Preparation and Applications of Organotypic Thymic Slice Cultures
 

Preparation and Applications of Organotypic Thymic Slice Cultures

Article DOI: 10.3791/54355-v • 10:10 min • August 6th, 2016
August 6th, 2016 •
*1,2, *1,2, 1,3
1Centre de Recherche, Hôpital Maisonneuve-Rosemont, 2Department of Microbiology, Infectiology and Immunology, Université de Montréal, 3Department of Medicine, Université de Montréal
* These authors contributed equally

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Resumo
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We describe the preparation of thymic slices that, in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. Thymic slices can also be adapted for the in situ analysis of thymocyte migration, localization, and signaling via immunofluorescence and two-photon microscopy.

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Keywords: Organotypic Thymic Slice Cultures T Cell Development Positive And Negative Selection Thymic Cortex And Medulla Thymocyte Subsets Thymic Microenvironment Mouse Thymus Dissection Agarose Embedding RPMI-1640 Media Cell Culture Inserts
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