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Measuring Lactase Enzymatic Activity in the Teaching Lab
 

Measuring Lactase Enzymatic Activity in the Teaching Lab

Article DOI: 10.3791/54377-v • 04:41 min • August 6th, 2018
August 6th, 2018 •
1, 1,2, 1, 1,3, 1, 1
1School of Pharmacy, University of Reading, 2Department of Molecular Neuroscience, UCL Institute of Neurology, 3School of Biological Sciences, Royal Hollway, University of London

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Resumo
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The enzymatic activity of lactase is essential for the catabolic processing of the disaccharide lactose. Here, the activity of lactase found in dietary supplements is assayed using a colorimetric assay. This provides students with an experimental platform for understanding the activity of lactase and enzyme kinetics.

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Keywords: Lactase Enzyme Activity Teaching Lab ONPG PBS Spectrophotometry Temperature Enzyme Kinetics
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