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Use of a Central Venous Line for Fluids, Drugs and Nutrient Administration in a Mouse Model of Critical Illness
 

Use of a Central Venous Line for Fluids, Drugs and Nutrient Administration in a Mouse Model of Critical Illness

Article DOI: 10.3791/55553-v • 09:17 min • May 2nd, 2017
May 2nd, 2017 •
1, 1, 1, 1, 1, 1
1Clinical Division and Laboratory of Intensive Care Medicine, Department of Cellular and Molecular Medicine, KU Leuven

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Resumo
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This protocol describes a centrally catheterized mouse model of prolonged critical illness. We combine the cecal ligation and puncture method to induce sepsis with the use of a central venous line for fluids, drugs and nutrient administration to mimic the human clinical setting.

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Central Venous Line Fluids Administration Drug Administration Nutrient Administration Mouse Model Critical Illness Cecal Ligation And Puncture CLP Technique Intensive Care Medicine Endocrine And Metabolic Alterations Reproducible Model Pathogenesis Venous Line Tip Preparation Micro-renathane Tubing Sesame Oil Immersion Tubing Stretching Catheter Construction Adhesive Glue Application Catheter Flushing Metal Wire Folding
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