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Journal / Genetics / 杆状病毒基因治疗应用的生产与纯化
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杆状病毒基因治疗应用的生产与纯化
 
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杆状病毒基因治疗应用的生产与纯化

Article DOI: 10.3791/57019-v • 06:11 min • April 9th, 2018
April 9th, 2018 •
1,2, 1,2,3, 1,2
1Division of Experimental Hematology and Cancer Biology, Cincinnati Children's Hospital Medical Center, 2University of Cincinnati College of Medicine, 3Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia

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在该协议中, 杆状病毒是通过瞬态转染杆状病毒质粒进入 Sf9 细胞并在无血清悬浮培养中扩增而产生的。用肝素亲和层析法纯化上清液, 进一步浓缩离心。该协议对用于基因治疗的杆状病毒的制备和纯化具有重要意义。

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遗传学 问题 134 杆状病毒 Sf9 细胞 bacmid 色谱 纯化 肝素柱 ultracentrifuge。
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