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Immunology and Infection

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JoVE Journal Immunology and Infection
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
 

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Article DOI: 10.3791/57104-v • 07:51 min • October 21st, 2022
October 21st, 2022 •
1,2,3, 1,2, 2, 1,2,4, 4,5, 1,2, 2, 1,2
1Vision Institute, Sorbonne University, UM 80 / INSERM, UMR_S 968 / CNRS, UMR_7210, 2Quinze Vingts National Ophthalmology Hospital, 3Laboratory of Ophthalmic Instrument Development, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, 4Laboratory for Optics and Biosciences (LOB), École polytechnique, 5LOA - ENSTA Paris, École polytechnique

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We describe use of full-field optical coherence microscopy as a method for high quality assessment of corneal donor stroma. This protocol can be used to identify features indicative of health or disease, and is aimed at improving the screening and selection of donor tissues, and hence the results of keratoplasty.

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Full-Field Optical Coherence Microscopy Histology-Like Analysis Stromal Features Corneal Grafts Screening Selection Corneal Donor Tissues Eye Banks Cornea Transplantation High Resolution Assessment Capability Closed Chamber Storage Medium Contamination Risk Maelle Vilbert PhD Graduate Sample Holder Silica Cover Slip Imaging Surface Air Bubbles Ophthalmic Gel Optical Gel Power Switch Green LED Imaging Stage Movable Tray Acquisition Software Sample Identifier
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