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JoVE Journal Developmental Biology
Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞
 
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Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞

Article DOI: 10.3791/57212-v • 07:18 min • April 19th, 2018
April 19th, 2018 •
*1, *2, 1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institutes of Health, 2Office of Science and Technology, National Institute of Arthritis, Musculoskeletal and Skin Diseases (NIAMS), National Institutes of Health
* These authors contributed equally

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卫星细胞的精确识别对于研究其在各种生理和病理条件下的功能至关重要。本文提出了一种基于免疫荧光染色的成人骨骼肌切片卫星细胞识别的协议。

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发育生物学 问题 134 骨骼肌 卫星细胞 Pax7 免疫荧光 干细胞 基底叶片
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