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JoVE Journal Genetics
Kvantitativ analyse af Alternative Pre-mRNA-Splicing i mus hjernen sektioner ved hjælp af RNA In Situ hybridisering Assay
 
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Kvantitativ analyse af Alternative Pre-mRNA-Splicing i mus hjernen sektioner ved hjælp af RNA In Situ hybridisering Assay

Article DOI: 10.3791/57889-v • 11:22 min • August 26th, 2018
August 26th, 2018 •
1,2, 1,3, 1, 2, 1,3, 1,3,4
1Department of Genetics and Genome Sciences, Case Western Reserve University, 2Department of Endocrinology, Dongfang Hospital of Beijing University of Chinese Medicine, 3Case Comprehensive Cancer Center, Case Western Reserve University, 4Center for RNA Science and Therapeutics, Case Western Reserve University

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En i situ hybridisering (ISH) protokol, der bruger korte antisense oligonukleotider til at registrere alternative pre-mRNA splejsning mønstre i mus hjernen sektioner er beskrevet.

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Genetik spørgsmålet 138 In situ hybridisering neurofibromatosis type 1 exon 23a mus hjernen alternativ splicing
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