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Qdot 标记蛋白与现场特异修饰的λ DNA 在单分子水平上的相互作用可视化
 
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Qdot 标记蛋白与现场特异修饰的λ DNA 在单分子水平上的相互作用可视化

Article DOI: 10.3791/57967-v • 08:56 min • July 17th, 2018
July 17th, 2018 •
*1,2, *1, 1,2, 1,2
1State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 2Savaid Medical School, University of Chinese Academy of Sciences
* These authors contributed equally

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在这里, 我们提出了一个协议, 研究 DNA 蛋白相互作用的全内反射荧光显微镜 (TIRFM) 使用一个网站专门修改的λ DNA 基底和量子点标记蛋白。

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生物学 问题 137 单分子成像 DNA-蛋白质相互作用 荧光探针 量子点 全内反射荧光显微镜 λ DNA 站点特定修饰
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