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Journal / Cancer Research / 磷酸浓缩结合无标签定量质谱法研究前列腺癌的…
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Cancer Research

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JoVE Journal Cancer Research
磷酸浓缩结合无标签定量质谱法研究前列腺癌的 Phosphoproteome
 
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磷酸浓缩结合无标签定量质谱法研究前列腺癌的 Phosphoproteome

Article DOI: 10.3791/57996-v • 12:23 min • August 2nd, 2018
August 2nd, 2018 •
*1,2, *3, 4, 5, 1,2,3,6,7
1Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, 2Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, 3Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, 4Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, 5Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 6Pharmacology, Rutgers Robert Wood Johnson Medical School, 7Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey
* These authors contributed equally

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本协议描述了从前列腺癌细胞系或组织中提取和丰富磷酸肽持的过程,通过质谱法蛋白质组学分析 phosphoproteome。

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癌症研究 问题 138 前列腺癌 质谱 蛋白质组学 phosphoproteomics 磷酸化 激酶 细胞信号
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