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Immunology and Infection

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JoVE Journal Immunology and Infection
Antigenic Liposomes for Generation of Disease-specific Antibodies
 

Antigenic Liposomes for Generation of Disease-specific Antibodies

Article DOI: 10.3791/58285-v • 10:31 min • October 25th, 2018
October 25th, 2018 •
*1, *2,3, *3,4, 5, 6, 3,4, 5,7
1Janssen R&D, 2Department of Microbiology and Immunology, University of North Carolina, 3UNC Food Allergy Initiative, University of North Carolina, 4Department of Pediatrics, University of North Carolina, 5Department of Chemistry, University of Alberta, 6Department of Molecular Medicine, Scripps Research Institute, 7Department of Medical Microbiology and Immunology, University of Alberta
* These authors contributed equally

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Resumo
Automatic Translation

Described is the preparation of antigenic liposomal nanoparticles and their use in stimulating B-cell activation in vitro and in vivo. Consistent and robust antibody responses led to the development of a new peanut allergy model. The protocol for generating antigenic liposomes can be extended to different antigens and immunization models.

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Keywords: Antigenic Liposomes Disease-specific Antibodies Immunology Allergy Mouse Model Autoimmunity Liposomal Nanoparticles Heterobifunctional Crosslinker SPDP Dithiothreitol DSPE-PEG2000-Maleimide Lipid-linked Protein Lyophilization
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