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Journal / Neuroscience / Transplantation of Chemogenetically Engineered…
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Neuroscience

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JoVE Journal Neuroscience
Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains
 

Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains

Article DOI: 10.3791/59568-v • 06:09 min • August 26th, 2019
August 26th, 2019 •
1,2, 3, 3, 1
1Nervous System Development and Homeostasis Laboratory, The Francis Crick Institute, 2Neuroscience Centre, Biomedical Sciences Research Centre "Al. Fleming", 3Centre for Developmental Neurobiology, King's College London

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Here we present a protocol, designed to use chemogenetic tools to manipulate the activity of cortical interneuron progenitors transplanted into the cortex of early postnatal mice.

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Transplantation Chemogenetically Engineered Cortical Interneuron Progenitors Early Postnatal Mouse Brains Intrinsic Activity Maturation Targeting Manipulating Cellular Properties Scientific Community Protocol Handling Samples Equipment Visual Demonstration Petri Dishes Agarose PBS Olfactory Bulbs Solidify Carve Gel Microtome Base Surgical Blade Vibrating Blade Microtome
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