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Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands
 

Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands

Article DOI: 10.3791/59602-v • 07:15 min • June 11th, 2019
June 11th, 2019 •
1, 1, 1
1Department of Molecular Medicine, The Scripps Research Institute

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Resumo
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The lacrimal gland (LG) has two cell types expressing α-smooth muscle actin (αSMA): myoepithelial cells (MECs) and pericytes. MECs are of ectodermal origin, found in many glandular tissues, while pericytes are vascular smooth muscle cells of endodermal origin. This protocol isolates MECs and pericytes from murine LGs.

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Keywords: Myoepithelial Cells Pericytes Smooth Muscle Cells Exocrine Glands Tamoxifen Lacrymal Gland Parotid Gland Cell Isolation Cell Labeling Cell Suspension Cell Digestion Cell Culture Gene Expression
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