Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / An In Vitro Protocol for Evaluating MicroRNA…
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
 

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Article DOI: 10.3791/59628-v • 09:45 min • May 21st, 2019
May 21st, 2019 •
1, 1, 1, 1
1Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University

Capítulos

Resumo
Automatic Translation

This protocol uses a probe-based real-time polymerase chain reaction (PCR), a sulforhodamine B (SRB) assay, 3’ untranslated regions (3’ UTR) cloning, and a luciferase assay to verify the target genes of a miRNA of interest and to understand the functions of miRNAs.

Tags

Keywords: MicroRNA MicroRNA Target In Vitro Protocol Tumor Cells Cell Viability Assay Transfection MicroRNA Mimic MicroRNA-107
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter