Isolation og kultur af primære aorta endotelceller fra miniature grise

Article DOI: 10.3791/59673-v • 06:38 min • August 14th, 2019

August 14th, 2019 •

Yanli Zhao^1,2,3, Chengjiang Zhao⁴, David K.C. Cooper⁵, Ying Lu², Kewang Luo⁶, Huiyun Wang³, Pengfei Chen³, Changchun Zeng³, Shaodong Luan¹, Lisha Mou², Hanchao Gao^1,2,3

¹Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ²Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, ³Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁴Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, ⁵Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, ⁶People's Hospital of Longhua

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Der er beskrevet en effektiv enzymatisk metode til isolering af primære svin aorta endotheliale celler (pAECs) fra miniature grise. De isolerede primære pAECs kan anvendes til at undersøge immun-og koagulations reaktionen i xenotransplantation.

Isolation og kultur af primære aorta endotelceller fra miniature grise

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