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Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon
 

Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon

Article DOI: 10.3791/59862-v • 06:38 min • October 29th, 2021
October 29th, 2021 •
1,2, 4, 1,3, 1,3, 1, 1, 4, 1, 1,2, 1
1Institute of Marine Research, 2Department of Biological Sciences, University of Bergen, 3National Food Institute, Technical University of Denmark, 4Metal metabolism group, Nutritional Sciences Division, King's College London

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This article explains in detail a systematic approach to assess micro-mineral availability in Atlantic salmon. The methodology includes tools and models with increasing biological complexity: (1) chemical speciation analysis, (2) in vitro solubility, (3) uptake studies in cell lines, and (4) in vivo fish studies. 

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Mineral Availability Fish Feeds Complementary Methods Zinc Atlantic Salmon Solubility Micro-metals Chemical Forms Sources Radionuclide Suite Ground Feed Samples Zinc Radiotracer Buffer Aliquots Amino Acids Freshwater Intestinal Luminal Buffer Rotary Spinner Centrifuge Soluble Fractions Non-soluble Fractions Gamma Teller Counts Per Minute Rainbow Trout-derived Intestinal Cell Line EDTA Solution Trypsin Solution L-15 Medium FBS
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