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JoVE Journal Developmental Biology
An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
 

An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons

Article DOI: 10.3791/60031-v • 08:02 min • October 19th, 2019
October 19th, 2019 •
*1, *1,2, *1, 1
1School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 2Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen
* These authors contributed equally

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Resumo
Automatic Translation

In this report, we describe a simple protocol for studying neurite outgrowth in embryonic rat cortical neurons by co-transfecting with EGFP and the protein of interest.

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Keywords: EGFP Neurite Outgrowth Primary Neurons Transfection Neural Regeneration Brain Trauma Neurodegenerative Diseases Poly-D-Lysine Sprague Dawley Rat Cerebral Hemispheres Cortex Trypsin EDTA Maintenance Medium
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