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Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells
 

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells

Article DOI: 10.3791/60170-v • 12:02 min • November 11th, 2021
November 11th, 2021 •
1,2, 1,2,3, 2, 1,2,3, 1,2,3, 4,5,6
1Department of Pharmacology and Toxicology, University of Louisville, 2Experimental Therapeutics Program, Brown Cancer Center, University of Louisville, 3Department of Medicine, University of Louisville, 4Department of Surgery, University of Louisville, 5Immuno-Oncology Program, Brown Cancer Center, University of Louisville, 6Department of Microbiology and Immunology, University of Louisville

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This study describes a method to isolate exosome-enriched extracellular vesicles carrying immune-stimulatory granulocyte macrophage colony-stimulating factors from embryonic stem cells.

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Keywords: Exosome Extracellular Vesicles GM-CSF Embryonic Stem Cells Immune Response Ultracentrifugation Cell Culture Trypsin Gelatin Coating Cell Counting Cell Passaging Cell Supernatant Collection
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