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Immunology and Infection

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JoVE Journal Immunology and Infection
Måling af fagocytose af Aspergillus fumigatus Conidia af humane leukocytter ved hjælp af strømnings cytometri
 
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Måling af fagocytose af Aspergillus fumigatus Conidia af humane leukocytter ved hjælp af strømnings cytometri

Article DOI: 10.3791/60397-v • 09:43 min • December 7th, 2019
December 7th, 2019 •
1,2, 2, 1, 1,2, 1,2, 3, 2, 1,2
1Infections in Hematology and Oncology, Leibniz Institute for Infection Biology and Natural Product Research, 2Department for Hematology and Medical Oncology, Jena University Hospital, 3Institute for Transfusion Medicine, Jena University Hospital

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Denne protokol giver en hurtig og pålidelig metode til kvantitativt at måle fagocytose af Aspergillus fumigatus conidia af humane primære fagocytter ved hjælp af flow cytometri og at diskriminere fagocytose af conidia fra simpel vedhæftning til leukocytter.

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Immunologi og infektion fagocytose flow cytometri Aspergillus fumigatus CONIDIA FITC anti-FITC antistof aspergillose
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