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JoVE Journal Genetics
Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR
 

Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR

Article DOI: 10.3791/60511-v • 08:01 min • November 27th, 2019
November 27th, 2019 •
1, 1, 2, 3, 4, 5, 2, 1, 1
1Division of Immunology and Allergy, Lausanne University Hospital, 2Department of Fundamental Neuroscience, University of Lausanne, 3Department of Biochemistry, Erasmus Medical Center, 4Theoretical Biology and Biophysics Group, Los Alamos National Laboratories, 5Department of Biochemistry, University of Lausanne

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RNA hairpins and loops can function as primers for reverse transcription (RT) in absence of sequence-specific primers, interfering with the study of overlapping antisense transcripts. We have developed a technique able to identify strand-specific RNA, and we have used it to study HIV-1 antisense protein ASP.

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Keywords: HIV-1 Antisense Protein (ASP) RNA Transcripts Strand-specific RT-PCR Sense And Antisense Sequences Biotinylated Primers RNA Denaturation Anti-sense CDNAs Overlapping Genes Quantification DNase Treatment Reverse Transcription Endogenous RT Controls Reaction Mixture Magnetic Beads Washing And Binding Buffer
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