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Immunology and Infection

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JoVE Journal Immunology and Infection
Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
 

Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

Article DOI: 10.3791/60903-v • 07:26 min • March 4th, 2020
March 4th, 2020 •
1, 1
1Institute for Biomedical Sciences, Georgia State University

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We describe here a method for growth of Neisseria gonorrhoeae in metal-restricted liquid medium to facilitate the expression of genes for metal uptake. We also outline downstream experiments to characterize the phenotype of gonococci grown in these conditions. These methods may be adapted to be suitable for characterization of metal-responsive genes in other bacteria.

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Keywords: Metal-limited Growth Neisseria Gonorrhoeae Metal-responsive Genes Metal Acquisition Host Ligands Nutritional Immunity Metal Restricted Conditions Bacterial Growth CDM Klett Colorimeter Metal Loaded Proteins Transferrin Dialysis 96 Well Microplate Optical Density
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