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JoVE Journal Developmental Biology
Generering og utvidelse av humane kardiomyocytter fra pasientens perifere blodmonnukleære celler
 
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Generering og utvidelse av humane kardiomyocytter fra pasientens perifere blodmonnukleære celler

Article DOI: 10.3791/62206-v • 05:38 min • February 12th, 2021
February 12th, 2021 •
1,2, 3, 1,2, 1,2,4, 1,2,5, 3, 1,2,5,6
1Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, 2The Heart Center, Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology, The Ohio State University College of Medicine, 4Department of Anatomy, The Ohio State University College of Medicine, 5MCDB Graduate Program, The Ohio State University, 6Department of Pediatrics, The Ohio State University College of Medicine

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Her presenterer vi en protokoll for robust å generere og utvide humane kardiomyocytter fra pasientens perifere mononukleære celler i blodet.

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Utviklingsbiologi utgave 168 perifere mononukleære celler i blod PBMCer menneskeskapte pluripotente stamceller iPSCer hjertedifferensiering iPSC-reprogrammering kardiomyocyttutvidelse.
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