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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
 

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Article DOI: 10.3791/63525-v • 08:30 min • May 10th, 2022
May 10th, 2022 •
*1, *2, 3, 4, 4, 4, 1
1Otolaryngological Department of Tangdu Hospital, Fourth Military Medical University, 2Institute of Medical Research, Northwestern Polytechnical University, 3Orthopedic Department of Tangdu Hospital, Fourth Military Medical University, 4Department of Immunology, Fourth Military Medical University
* These authors contributed equally

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Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.

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Keywords: Innate Lymphoid Cells ILC2 Nasal Mucosa Mouse Flow Cytometry Allergic Rhinitis Ovalbumin Tissue Digestion Cell Isolation
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