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Journal / Immunology and Infection / Isolation of Uterine Innate Lymphoid Cells for…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
 

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Article DOI: 10.3791/62670-v • 09:02 min • October 14th, 2021
October 14th, 2021 •
*1,2, *1,2, *1,2, 1,2, 1, 1,2
1Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre, University of Cambridge School of Clinical Medicine, 2Centre for Trophoblast Research, University of Cambridge
* These authors contributed equally

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This is a method to isolate uterine lymphoid cells from both pregnant and non-pregnant mice. This method can be used for multiple downstream applications such as FACS phenotyping, cell sorting, functional assays, RNA-seq, and proteomics. The protocol here demonstrates how to phenotype group 1 uterine innate lymphoid cells by flow cytometry.

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Isolation Uterine Innate Lymphoid Cells Flow Cytometry Composition Functional Characteristics Lymphocyte Subpopulations Uterine Tissue Pregnant Animals Non-pregnant Animals Isolation Method Surface Expression Of Protein Cell Viability Functionality Subsequent Applications Fetus Removal Leukocyte Collection Antibody Staining Steps Sterile Petri Dish Fat Removal Tissue Drying Prevention Implantation Site Dissection Collection Media Enzymatic Digestion Mix Incubation Temperature And Time
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