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JoVE Journal Cancer Research
An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue
 

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

Article DOI: 10.3791/64499-v • 10:33 min • February 3rd, 2023
February 3rd, 2023 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1The First School of Clinical Medicine, Gannan Medical University, 2Laboratory Medicine, First Affiliated Hospital of Gannan Medical University

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Here we describe the enrichment of small extracellular vesicles derived from liver cancer tissue through an optimized differential ultracentrifugation method.

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Enrichment Method Small Extracellular Vesicles Liver Cancer Tissue Isolation Purity Ultracentrifugation Technology Simple Convenient Easy To Operate Methodologic Support Tissue Sample Mince Digestive Solution Transference Decoloring Shaker Incubate Dissociation SEVs Phosphatase Inhibitor Protease Inhibitor Solution Digestion Cell Strainer Centrifuge Filtrate Supernatant
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