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Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice
 

Bioluminescent Monitoring of Graft Survival in an Adoptive Transfer Model of Autoimmune Diabetes in Mice

Article DOI: 10.3791/64836-v • 10:03 min • November 18th, 2022
November 18th, 2022 •
1, 2, 2, 1
1Section for Islet Cell and Regenerative Biology, Joslin Diabetes Center, Harvard Medical School, 2Section for Immunology, Joslin Diabetes Center, Harvard Medical School

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This protocol describes a straightforward and minimally invasive method for transplanting and imaging NIT-1 cells in non-obese diabetic (NOD)-severe combined immunodeficient mice challenged with splenocytes purified from spontaneously diabetic NOD mice.

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Keywords: Bioluminescent Monitoring Graft Survival Adoptive Transfer Autoimmune Diabetes Mice Cell Culture Transplant Imaging Tail Vein Injection Isoflurane Subcutaneous Injection Cell Suspension
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