وسحق العصب البصري النموذجي إصابة الفئران لدراسة الخلايا الشبكية بقاء العقدة
Summary
This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma.
Abstract
Injury to the optic nerve can lead to axonal degeneration, followed by a gradual death of retinal ganglion cells (RGCs), which results in irreversible vision loss. Examples of such diseases in human include traumatic optic neuropathy and optic nerve degeneration in glaucoma. It is characterized by typical changes in the optic nerve head, progressive optic nerve degeneration, and loss of retinal ganglion cells, if uncontrolled, leading to vision loss and blindness.
The optic nerve crush (ONC) injury mouse model is an important experimental disease model for traumatic optic neuropathy, glaucoma, etc. In this model, the crush injury to the optic nerve leads to gradual retinal ganglion cells apoptosis. This disease model can be used to study the general processes and mechanisms of neuronal death and survival, which is essential for the development of therapeutic measures. In addition, pharmacological and molecular approaches can be used in this model to identify and test potential therapeutic reagents to treat different types of optic neuropathy.
Here, we provide a step by step demonstration of (I) Baseline retrograde labeling of retinal ganglion cells (RGCs) at day 1, (II) Optic nerve crush injury at day 4, (III) Harvest the retinae and analyze RGC survival at day 11, and (IV) Representative result.
Protocol
Discussion
The optic nerve crush injury murine model is useful to study the process of RGC death and survival. This model is also often used to investigate the effects of different reagents and genes on RGC apoptosis and survival. One advantage of this model is that it has a high degree of reproducibility with minimal variations.
However, special care is required in several steps in this model. First, when making the optic nerve crush injury, it is essential not to use too much force and not to crush for…
Disclosures
The authors have nothing to disclose.
Acknowledgements
Our research is supported by the Intramural Research Program of the NIH, National Eye Institute.
Materials
| Material Name | Type | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Stereotaxic apparatus | ASI Instruments | SAS-4100 | ||
| Dissecting microscope | World Precision Instruments | PZMIV-BS | ||
| Scalpel handle | Fine Science Tools | 10003-12 | ||
| Scalpel Blades #15 | Fine Science Tools | 10015-00 | ||
| Microdrill | Fine Science Tools | 18000-17 | ||
| Suture needle | Fine Science Tools | 12050-03 | ||
| Spring scissors | Fine Science Tools | 16144-13 | ||
| Dumont #5 forceps | Fine Science Tools | 11252-50 | ||
| Hamilton syringe | Hamilton Company | 88400 | ||
| Gauze sponges | Office Depot | 674889 | ||
| Fluorochrome | Sigma | 39286 | ||
| Alcohol | Sigma | 459844 | 70% | |
| H2O2 | Sigma | H3410 | 3% |
References
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