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Chapters
- 00:05Title
- 01:13Introduction
- 01:42Isolation of Heart Valve Leaflets
- 03:03Isolation of Endothelial Layer
- 05:22Isolation of Side Specific Endothelial Layer
- 08:03Isolation of Interstitial Cells
- 09:07Isolation of Valvular Endothelial Cells Representative Images
- 10:30Conclusion
Wir stellen eine Methode zur Isolierung und Kultivierung von reinen Populationen von Herzklappen-Endothelzellen (VEC). VEC von beiden Seiten der Höcker oder Prospekt isoliert werden können und unmittelbar nach ist zugrundeliegenden interstitiellen Zellen (VIC) Isolierung einfach.
Tags
IsolationValvular Endothelial CellsHeart ValvesUnidirectional Blood FlowCardiovascular SystemMechanical StressesValvular Endothelial CellsInterstitial CellsRepair And RemodelIn Vitro ExperimentationIsolation And CultureDirect IsolationSide Specific IsolationCultureVerification Of Pure PopulationsEnzymatic DigestionGentle Swab Scraping TechniqueSurface CellsCentrifuged Into A PelletResuspended And PlatedCollagen I MatrixVEC PhenotypeContact Inhibited GrowthEndothelial Specific Markers (PECAM1/CD31)Von Willebrand Factor (vWF)Alpha-smooth Muscle Actin (α-SMA)Functional Characteristics
