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Chapters
- 00:05Title
- 01:13Introduction
- 01:42Isolation of Heart Valve Leaflets
- 03:03Isolation of Endothelial Layer
- 05:22Isolation of Side Specific Endothelial Layer
- 08:03Isolation of Interstitial Cells
- 09:07Isolation of Valvular Endothelial Cells Representative Images
- 10:30Conclusion
Ofrecemos un método para aislar y cultivar las poblaciones puras de células endoteliales de la válvula del corazón (VEC). VEC puede ser aislada de uno y otro lado de la cúspide o el prospecto e inmediatamente después, las células intersticiales de base (CIV) aislada es muy sencillo.
Tags
IsolationValvular Endothelial CellsHeart ValvesUnidirectional Blood FlowCardiovascular SystemMechanical StressesValvular Endothelial CellsInterstitial CellsRepair And RemodelIn Vitro ExperimentationIsolation And CultureDirect IsolationSide Specific IsolationCultureVerification Of Pure PopulationsEnzymatic DigestionGentle Swab Scraping TechniqueSurface CellsCentrifuged Into A PelletResuspended And PlatedCollagen I MatrixVEC PhenotypeContact Inhibited GrowthEndothelial Specific Markers (PECAM1/CD31)Von Willebrand Factor (vWF)Alpha-smooth Muscle Actin (α-SMA)Functional Characteristics
