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流式细胞仪定量测定GLUT4转位到质膜
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
流式细胞仪定量测定GLUT4转位到质膜
DOI:
10.3791/2429-v
•
05:39 min
•
November 07, 2010
•
Shyny Koshy
,
Parema Alizadeh
,
Lubov T. Timchenko
,
Christine Beeton
1
Department of Molecular Physiology and Biophysics
,
Baylor College of Medicine
Chapters
00:00
Title
01:15
Cell Staining
04:05
Data Aquisition
04:39
Upregulation of Surface GLUT-4 Receptor in Response to Insulin on Myoblasts from Healthy or Insulin-resistant Individuals
05:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种快速的技术量化从细胞质GLUT4转位的流式细胞仪检测细胞质膜。
Tags
Quantitative Measurement
GLUT4 Translocation
Plasma Membrane
Flow Cytometry
Glucose
Energy Source
Blood Concentration
Insulin Release
Type-2 Diabetes
Obesity
Insulin Resistance
Glucose Homeostasis
Mechanisms
Treatment Strategies
GLUT Family
Glucose Transporters
GLUT4 Transporter
Skeletal Muscle
Insulin Receptor
Vesicles
Cytoplasm
Immunocytochemistry
Fluorophore-conjugated Antibodies
Technique
Quantify
Total Amounts Of GLUT4 Translocation
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