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Chapters
- 00:05Title
- 01:37Preparation of Cell Cytosol Containing Functional Glucocorticoid Receptor
- 03:04Immunoabsorption of GR from the Cytosol and Dissociation of hsp90
- 05:16Reconstitution of GR-hsp90 Heterocomplex using Molecular Chaperones, Cofactors, and ATP
- 06:17Analysis of Reconstituted Protein Complexes and Ligand Binding Activity
- 08:26Representative GR-hsp90 Heterocomplex Formation and Steroid Binding Activity
- 10:04Conclusion
Tags
Biochemical ReconstitutionSteroid ReceptorHsp90 Protein ComplexesLigand BindingMolecular ChaperoneEukaryotic Signaling ProteinsTranscription FactorsProtein KinasesCell CyclingTumorigenesisApoptosisSignaling PathwaysGlucocorticoid Receptor (GR)Immunoprecipitation AssayIn Vitro Reconstitution MethodHsp90 Functional ActivitySteroid Receptor Ligand BindingMolecular Chaperone Cofactor Requirements
