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从核衣壳的病毒DNA的制备
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Viral DNA from Nucleocapsids
DOI:

12:45 min

August 16, 2011

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Chapters

  • 00:05Title
  • 01:34Day 1: Viral Infection and Preparation of Buffers
  • 02:56Day 2, Phase 1: Cell Lysis and Ultracentrifugation
  • 04:01Day 2, Phase 2: Ultracentrifugation
  • 07:35“Ghost” DNA Precipitation
  • 09:56Representative Results
  • 11:51Conclusion

Summary

Automatic Translation

我们描述了从被感染的细胞中分离高纯度的疱疹病毒衣壳DNA的过程。从溶液中捕获的是最后的DNA浓度和纯度高,使得它非常适合高通量测序,高保真PCR反应,并产生新的病毒重组体转染。

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