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Chapters
- 00:05Title
- 01:37PORN-H/Laminin Coating of Dishes and Coverslips
- 02:33Lectin Coating of the Purification Plate
- 03:20Motoneuron Culture Medium
- 04:32Spinal Cord Dissection
- 05:35Enrichment of Motoneurons by Lectin-based Purification
- 07:28Results: Lectin-based Isolation and Characterization of Mouse Embryonic Motoneurons
- 08:46Conclusion
Omurilik fare embriyonik motor nöronlar izole alternatif bir yol tarif edilir. Bu yöntem, lektin düşük afinite sinir büyüme faktörü reseptör p75NTR bağlamak için aslında dikkate alır. Bu lektin tabanlı preplating p75NTR karşı spesifik antikor ile benzer bir arınma sağlar.
Tags
Lectin-based IsolationCultureMouse Embryonic MotoneuronsSpinal MotoneuronsNeuroepithelial CellsNkx6.1Precursor CellsPostmitotic StagesDendritesAxonsTranscription FactorsIslet 1/2Innervate MusclesHigh Affinity ReceptorsBrain Derived Neurotrophic Factor (BDNF)Neurotrophin-3 (NT-3)Tropomyosin-related Kinase B And C (TrkBTrkC)Tropomyosin-related Kinase A (TrkA)Low Affinity Neurotrophin Receptor P75NTRIsolation TechniquesSpecific Antibodies (panning)
