A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:15Loading Cells with Calcium Indicators
- 02:23Microinjecting Cells
- 03:42Co-immunofluorescence Studies
- 04:49Fast Calcium Imaging and Data Analysis
- 06:28Dissecting the Timing of Ca++ Fluxes Facilitates Evaluation of Their Impact on Cellular Events
- 11:22Conclusion
Vi presenterer en metode for å isolere rask (mikrosekund) kalsium hendelser fra tregere flukser i levende celler ved hjelp av laser scanning konfokalmikroskopi. Metoden måler fluorescensintensitet svingninger av kalsium indikatorer ved registrering linje skanninger av flere hundre piksler i en celle. Histogram analyse tillater oss å isolere tidsskalaer på forskjellige kalsium flukser.
Tags
Calcium FluxesCardiomyocytesMeasuringFastCa2+ ActivityPhospholipase Cβ- Gαq PathwayAcetylcholineCaveolaeEntrapmentGαqProlonged Ca2+ SignalsDynamic Calcium ResponsesFluorescent Ca2+ IndicatorCa2+ GreenFluorescence Emission IntensityLine-scan ModeLaser Scanning Confocal MicroscopeTime CourseMicrosecond Time ScaleExcel AnalysisSigmaplot AnalysisFlux Rate
