A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:12Set-up Prior to Harvest
- 02:09Tissue Harvest
- 04:03Tissue Dissociation
- 05:23Neuron Trituration
- 06:42Cell Plating
- 08:43Immunofluorescence Results with Hippocampal Neuronal Cultures
- 09:58Conclusion
Tags
IsolationCultureHippocampal NeuronsPrenatal MicePrimary CulturesRatMurineNeurobiologyCellular MechanismsCellular TraffickingCellular StructureProtein LocalizationBiochemical TechniquesMemoryLearningContaminationBrain Cell TypesSpecific MediaNeuron GrowthDissection TechniquesEmbryonic Day 19 (E19)Mouse PupsTrypsinMechanical DisruptionPipettes
