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Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer’s Vesicle
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Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer’s Vesicle

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer’s Vesicle

DOI:
10.3791/50038-v

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08:11 min

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March 31, 2013

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, ,
1Department of Cell and Developmental Biology, State University of New York,Upstate Medical University, 2Department of Neurobiology and Anatomy, Eccles Institute of Human Genetics,University of Utah

Chapters

  • 00:05Title
  • 01:49Stage-specific Injection of Morpholinos (MO)
  • 03:10Selecting Injected Embryos for Analysis
  • 04:16Mounting Embryos and Injection of Fluorescent Beads to Analyze Fluid Flow in Kupffer’s Vesicle
  • 05:56Visualization and Analysis of KV Fluid Flow
  • 06:38Representative Kupffer’s Vesicle Fluid Flow Results
  • 07:45Conclusion

Summary

Automatic Translation

Automatic Translation

Cilia-generated fluid flow in Kupffer’s Vesicle (KV) controls left-right patterning of the zebrafish embryo. Here, we describe a technique to modulate gene function specifically in KV cells. In addition, we show how to deliver fluorescent beads into KV to visualize fluid flow.

Tags

Keywords: Gene FunctionCiliaKupffer’s VesicleLeft-right AsymmetryFluid FlowZebrafishDorsal Forerunner CellsMorpholino KnockdownVideomicroscopy

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