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Analyse der Genfunktion und Visualisierung von Cilia-Generated Fluid Flow in Kupffer-Vesicle
JoVE Journal
Biology
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JoVE Journal Biology
Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer’s Vesicle
DOI:

08:11 min

March 31, 2013

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Chapters

  • 00:05Title
  • 01:49Stage-specific Injection of Morpholinos (MO)
  • 03:10Selecting Injected Embryos for Analysis
  • 04:16Mounting Embryos and Injection of Fluorescent Beads to Analyze Fluid Flow in Kupffer’s Vesicle
  • 05:56Visualization and Analysis of KV Fluid Flow
  • 06:38Representative Kupffer’s Vesicle Fluid Flow Results
  • 07:45Conclusion

Summary

Automatic Translation

Cilia-generated Fluidströmung in Kupffer-Vesikel (KV) steuert links-rechts Strukturierung des Zebrafisch-Embryos. Hier beschreiben wir ein Verfahren zur Modulation Genfunktion spezifisch in Zellen KV. Darüber hinaus zeigen wir, wie fluoreszierende Kügelchen in KV liefern Strömung zu visualisieren.

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