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基因功能分析和可视化的纤毛产生的流体流动的在枯否囊泡中

JoVE Journal
Biology

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JoVE Journal Biology

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer’s Vesicle

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基因功能分析和可视化的纤毛产生的流体流动的在枯否囊泡中

DOI:

10.3791/50038-v

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08:11 min

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March 31, 2013

•

Guangliang Wang, H. Joseph Yost, Jeffrey D. Amack

¹Department of Cell and Developmental Biology, State University of New York,Upstate Medical University, ²Department of Neurobiology and Anatomy, Eccles Institute of Human Genetics,University of Utah

Chapters

00:05Title
01:49Stage-specific Injection of Morpholinos (MO)
03:10Selecting Injected Embryos for Analysis
04:16Mounting Embryos and Injection of Fluorescent Beads to Analyze Fluid Flow in Kupffer’s Vesicle
05:56Visualization and Analysis of KV Fluid Flow
06:38Representative Kupffer’s Vesicle Fluid Flow Results
07:45Conclusion

Summary

Automatic Translation

纤毛产生的流体流动在枯否囊泡（KV）控制的斑马鱼胚胎的左右模式。在这里，我们描述了一种技术，调节基因的功能，特别是在KV细胞。此外，我们展示了如何提供KV可视化流体流动的荧光珠。

Tags

Keywords: Gene Function Cilia Kupffer’s Vesicle Left-right Asymmetry Fluid Flow Zebrafish Dorsal Forerunner Cells Morpholino Knockdown Videomicroscopy

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