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Summary
This protocol describes the use of the Ramsay assay to measure fluid secretion rates from isolated Malpighian (renal) tubules from Drosophila melanogaster. In addition, the use of ion-specific electrodes to measure sodium and potassium concentrations in the secreted fluid, allowing calculation of transepithelial ion flux, is described.
Tags
Keywords: Ramsay Assay Fluid Secretion Ion Flux Drosophila Melanogaster Malpighian Tubule Epithelial Ion Transport Transepithelial Fluid Ion-specific Electrodes Sodium Potassium Genetics Molecular Mechanisms Homeostasis
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An Introduction to the Human Body
Overview of Anatomy and Physiology
Structural Organization of the Human Body
Functions of Human Life
Requirements for Human Life
Homeostasis
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Medical Imaging
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Elements and Atoms: The Building Blocks of Matter
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