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成像APP的细胞内运输与光活化GFP
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JoVE Journal Biology
Imaging the Intracellular Trafficking of APP with Photoactivatable GFP
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成像APP的细胞内运输与光活化GFP

DOI:
10.3791/53153-v

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07:55 min

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October 17, 2015

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1Department of Physiology and Pharmacology, Robarts Research Institute,Western University, 2Department of Clinical Neurological Sciences,Western University

Chapters

  • 00:05Title
  • 00:54Cell Transfection and Microscope Stage Preparation
  • 01:52Photoactivation of ROIs over the Golgi
  • 03:20Determining the Downstream Compartment
  • 04:00Ensuring Accuracy of Photoactivation within the Golgi
  • 05:42Results: Photoactivatable Intracellular Trafficking Analysis Using βAPP-paGFP Photoactivation
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

而细胞表面蛋白的传输相对容易地研究，可视化细胞内蛋白质的贩卖是困难得多。在这里，我们使用结合光活化绿色荧光蛋白的结构和表现出的方​​法来准确地遵循从高尔基体向下游车厢淀粉样前体蛋白，并按照其清除。

Tags

Keywords: Amyloid Precursor Protein (APP)Beta-amyloid (Aβ)Alzheimer’s DiseasePhotoactivatable GFP (paGFP)Intracellular TraffickingGolgiEndosomesLysosomesSecretase InhibitorsPulse-chase Experiments

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