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İlaç Direnci İlgili Roman Splice türevlerini algılamak için RNA sıralamayı kullanarak<em> İn Vitro</em> Kanser Modelleri
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1Department of Pediatric Oncology/Hematology,VU University Medical Center, 2Department of Hematology,VU University Medical Center, 3Department of Medical Oncology,VU University Medical Center, 4Department of Clinical Genetics,VU University Medical Center, 5Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana,Universita’ di Pisa, 6Amsterdam Immunology and Rheumatology Center,VU University Medical Center, 7Princess Máxima Center for Pediatric Oncology, 8Cancer Pharmacology Lab, AIRC Start-Up Unit,University of Pisa, 9Institute of Nanoscience and Nanotechnology,CNR-Nano
Chapters
- 00:05Title
- 01:09Characterization of Drug Resistance Profiles through Cytotoxicity Assays
- 05:01RNA Isolation and Library Preparation for RNA-sequencing
- 05:55Validation of the Results by RT-PCR and qRT-PCR Assays
- 07:21Results: Detection of Differential Splicing Events in Chemotherapy-resistant Cell Lines Compared to Parental Wild-type by Using RNA-sequencing
- 09:07Conclusion
Burada katı tümörler ve hematolojik malignitelerde ilaç direnci üzerinde anormal yapıştırma etkisini araştıran amaçlayan bir protokol açıklar. Bu hedefe, biz RNA seq ile ebeveyn ve in vitro dirençli modellerin transkriptomik profillerini analiz ve aday genler doğrulamak için bir QRT-PCR tabanlı bir yöntem kurdu.
