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جهاز لأداء الهجرة خلية / شفاء الجروح في لوحة 96-حسنا
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
DOI:

05:46 min

March 07, 2017

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Chapters

  • 00:05Title
  • 00:59Introducing the Wounder Parts
  • 01:38Setting the Pin Holder Width
  • 02:05Adjusting the Guiding Bars
  • 02:49Calibrating the Pins
  • 03:23Scratching the Cell Monolayer
  • 04:44Results: Uniform Wound Width with Device
  • 05:13Conclusion

Summary

Automatic Translation

Here, we present a protocol to perform a semi-high throughput cell migration assay on a 96-well cell culture plate. This protocol is a fast, simple and economical method to create consistent scratch wounds on a cell monolayer.

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