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Journal
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Developmental Biology
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一种在96孔板表演细胞迁移/伤口愈合设备
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
Please note that all translations are automatically generated.
Click here for the English version.
一种在96孔板表演细胞迁移/伤口愈合设备
DOI:
10.3791/55411-v
•
05:46 min
•
March 07, 2017
•
Po Ying Poon
,
Patrick Ying Kit Yue
,
Ricky Ngok Shun Wong
1
Department of Biology
,
Hong Kong Baptist University
Chapters
00:05
Title
00:59
Introducing the Wounder Parts
01:38
Setting the Pin Holder Width
02:05
Adjusting the Guiding Bars
02:49
Calibrating the Pins
03:23
Scratching the Cell Monolayer
04:44
Results: Uniform Wound Width with Device
05:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个协议,以在96孔细胞培养板进行半高通量细胞迁移测定。该协议是建立在一个细胞单层一致划伤伤口快速,简便,经济的方法。
Tags
Cell Migration
Wound Healing
96-well Plate
In Vitro Assay
Cell Migration Quantification
Semi-high Throughput
Adjustable Wounding Pins
Guiding Bar
Pipet Tips
Hex Screw
Hex Socket Head Caps
Adjusting Rings
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