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一种在96孔板表演细胞迁移/伤口愈合设备

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Developmental Biology

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JoVE Journal Developmental Biology

A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

Please note that all translations are automatically generated. Click here for the English version.

一种在96孔板表演细胞迁移/伤口愈合设备

DOI:

10.3791/55411-v

•

05:46 min

•

March 07, 2017

•

Po Ying Poon, Patrick Ying Kit Yue, Ricky Ngok Shun Wong

¹Department of Biology,Hong Kong Baptist University

Chapters

00:05Title
00:59Introducing the Wounder Parts
01:38Setting the Pin Holder Width
02:05Adjusting the Guiding Bars
02:49Calibrating the Pins
03:23Scratching the Cell Monolayer
04:44Results: Uniform Wound Width with Device
05:13Conclusion

Summary

Automatic Translation

在这里，我们提出了一个协议，以在96孔细胞培养板进行半高通量细胞迁移测定。该协议是建立在一个细胞单层一致划伤伤口快速，简便，经济的方法。

Tags

Cell Migration Wound Healing 96-well Plate In Vitro Assay Cell Migration Quantification Semi-high Throughput Adjustable Wounding Pins Guiding Bar Pipet Tips Hex Screw Hex Socket Head Caps Adjusting Rings

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