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Studi struttura-funzione nel topo cellule staminali embrionali Uso ricombinasi mediata Cassette Scambio
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1Department of Biomedical Molecular Biology,Ghent University, 2Inflammation Research Center,VIB, 3Center for Medical Genetics,Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences,Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology,Monash University and Alfred Health Alfred Centre
Chapters
- 00:05Title
- 01:11The RMCE Principle
- 02:15Isolation of RMCE-compatible Knockout Mouse ES Cells
- 05:22Isolate RMCE-compatible Knockout ES Cells
- 07:29Generation of an RMCE-compatible Targeting Vector Using Recombination Cloning
- 09:25RMCE-mediated ES Cell Targeting of Rescue Constructs to the R26 Locus and Differentiation of ES Cells in Embryoid Bodies
- 12:41Results: Cystic EB Formation Using Recombinase Mediated Cassette Exchange
- 14:27Conclusion
Le proteine contengono spesso più domini che possono esercitare diverse funzioni cellulari. Gene knock-out (KO) non considerano questa diversità funzionale. Qui riportiamo un cambio cassette ricombinazione mediata (RMCE) approccio basato lo struttura-funzione in cellule staminali embrionali KO che consente la dissezione molecolare dei vari domini funzionali o varianti di una proteina.
