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激光 Microirradiation 研究体内细胞对简单复杂 DNA 损伤的反应
JoVE Journal
Genetics
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JoVE Journal Genetics
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
DOI:

10:44 min

January 31, 2018

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Chapters

  • 00:05Title
  • 01:25Transient Transfection
  • 04:09Titration of Laser Input Power
  • 05:40Quantitative Fluorescence Analysis of Immunostained or Live Cells
  • 06:54Cell Cycle and G1 Phase Synchronization
  • 07:46Results: In Vivo Cellular Responses to Complex DNA Damage
  • 09:27Conclusion

Summary

Automatic Translation

该协议的目的是描述如何使用激光 microirradiation 诱导不同类型的 dna 损伤, 包括相对简单的断链和复杂的损害, 研究 dna 损伤信号和修复因子在损伤部位的组装在体内.

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