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Adeno-associeret Virus-medieret transgen udtryk i genetisk definerede neuroner i rygmarven
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
DOI:

08:41 min

May 12, 2018

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Chapters

  • 00:04Title
  • 00:42Exposure, Identification, and Fixation of the Vertebrae at the Lumbar Spinal Cord Level
  • 04:08Preparation of the Injection Syringe
  • 05:25Intraspinal Injections
  • 07:12Results: Intraspinal Injection of AAV1-eGFP/AAV1-flex-eGFP
  • 08:14Conclusion

Summary

Automatic Translation

Intraspinal injektion af recombinase afhænger af rekombinant adeno-associeret virus (rAAV) kan bruges til at manipulere nogen genetisk mærket celletype i rygmarven. Her beskriver vi, hvordan du transduce neuroner i dorsal Hornet af lumbal rygmarven. Denne teknik gør det muligt for funktionelle forhør af undertypen manipulerede neuron.

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