A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:56Preparations
- 01:42Extraction of the Small Intestines from the Abdominal Cavity
- 03:11Fabricating Intestinal Loops and Injecting them with Pathogens
- 04:58Completing the Procedure and Collecting Results
- 06:44Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion
- 08:02Conclusion
Her præsenterer vi en protokol for at kirurgisk oprette intestinal sammenskrevne sløjfer i kylling små tarme. Denne procedure giver mulighed for sammenligning af flere Clostridium perfringens stammer virulens i situ i en enkelt vært. Denne metode reducerer markant antallet af kyllinger normalt nødvendigt for lignende i vivo eksperimenter.
