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Chapters
- 00:04Title
- 00:56Preparations
- 01:42Extraction of the Small Intestines from the Abdominal Cavity
- 03:11Fabricating Intestinal Loops and Injecting them with Pathogens
- 04:58Completing the Procedure and Collecting Results
- 06:44Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion
- 08:02Conclusion
Qui presentiamo un protocollo per creare chirurgicamente 'anse intestinali legati' in intestini di pollo. Questa procedura consente il confronto di virulenza in situ in un singolo host di più ceppi Clostridium perfringens . Questo metodo notevolmente riduce il numero di polli solitamente necessari per simili esperimenti in vivo .
