A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:56Preparations
- 01:42Extraction of the Small Intestines from the Abdominal Cavity
- 03:11Fabricating Intestinal Loops and Injecting them with Pathogens
- 04:58Completing the Procedure and Collecting Results
- 06:44Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion
- 08:02Conclusion
Aquí presentamos un protocolo quirúrgico crear «loops ligados intestinales» en intestino de pollo. Este procedimiento permite la comparación de virulencia en situ en un único host de varias cepas de Clostridium perfringens . Este método disminuye marcadamente el número de pollos generalmente necesarios para experimentos similares en vivo .
